Removal of Single-Stranded Extension using Mung Bean Nuclease (M0250)
New England Biolabs
Published: 2022-02-20 DOI: 10.17504/protocols.io.bb2riqd6
Abstract
This is the protocol for the removal of single-stranded extension using Mung Bean Nuclease (M0250).
Before start
Steps
1.
Suspend DNA (0.1μg/μl) in 1X Mung Bean Nuclease Buffer or 1X NEBuffer 1.1, 2.1 or CutSmart ®.
2.
Add 1.0 unit of Mung Bean Nuclease per μg DNA.
3.
Incubate at 30°C for 0h 30m 0s.
4.
Inactivate the enzyme by phenol/chloroform extraction or by addition of SDS to 0.01%.
5.
Recover the DNA by ethanol precipitation.
