Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing

Peter Ellis, Luiza Moore, Mathijs A. Sanders, Timothy M. Butler, Simon F. Brunner, Henry Lee-Six, Robert Osborne, Ben Farr, Tim H. H. Coorens, Andrew R. J. Lawson, Alex Cagan, Mike R. Stratton, Inigo Martincorena, Peter J. Campbell

Published: 2020-12-13 DOI: 10.1038/s41596-020-00437-6

laser-capture microdissection

low-input DNA sequencing

somatic mutations

genome sequencing

tissue processing

AI 解读

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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Somatic mutations accumulate in healthy tissues as we age, giving rise to cancer and potentially contributing to ageing. To study somatic mutations in non-neoplastic tissues, we developed a series of protocols to sequence the genomes of small populations
Introduction
Materials
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Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS

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Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing

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