Quick Decapitation for Paraffin Embedding
Haley Geertsma
Published: 2022-03-02 DOI: 10.17504/protocols.io.b5szq6f6
Abstract
This protocol is used to quickly decapitate mice and isolate their brain for paraffin embedding and microtome sectioning.
Steps
1.
Sedate mice with isoflurane then quickly decapitate.
2.
Extract the brain and incubate in 4% paraformaldehyde or 10% formalin for 72 hours
3.
Incubate brain in 70% ethanol and submit for paraffin embedding. Once embedded, section brains at 5μm.
