Purification of lysosomes using dextran-conjugated SPIONs

Pietro De Camilli, William Hancock-Cerutti, Arun Kumar Tharkeshwar, Shawn Ferguson

Published: 2022-04-02 DOI: 10.17504/protocols.io.bp2l61dr1vqe/v1

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Abstract

This method describes the usage of dextran-conjugated superparamagnetic iron nanoparticles (SPIONs) to purify a lysosome fraction from cultured cells.

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HC-Protocol-Lysosome-purification-SPIONS.docx

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