Purification, full-length sequencing and genomic origin mapping of eccDNA

Meng Wang, Yi Zhang, Yuangao Wang

Published: 2022-12-13 DOI: 10.1038/s41596-022-00783-7

eccDNA purification

3SEP

full-length sequencing

Nanopore

genomic origin mapping

AI 解读

Mapping the common gene networks that underlie related diseases

A nanobody-based strategy for rapid and scalable purification of human protein complexes

A general protocol for precise syntheses of ordered mesoporous intermetallic nanoparticles

Author Correction: m6A-SAC-seq for quantitative whole transcriptome m6A profiling

In vivo identification of astrocyte and neuron subproteomes by proximity-dependent biotinylation

Construction and use of an adaptive optics two-photon microscope with direct wavefront sensing

Simultaneous ribosome profiling of hundreds of microbes from the human microbiome

Global mapping of RNA G-quadruplexes (G4-RNAs) using G4RP-seq

Enzyme-free targeted DNA demethylation using CRISPR–dCas9-based steric hindrance to identify DNA methylation marks causal to altered gene expression

Minimally invasive longitudinal intravital imaging of cellular dynamics in intact long bone

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Extrachromosomal circular DNA (eccDNA) was discovered more than half a century ago. However, its biogenesis and function have just begun to be elucidated. One hurdle that has prevented our understanding of eccDNA is the difficulty in obtaining pure eccDNA
Introduction
Materials
Procedure
Anticipated results
Troubleshooting
Timing
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- References
References
ACKNOWLEDGMENTS

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Purification, full-length sequencing and genomic origin mapping of eccDNA

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