Protocol for Bulk Water Enrichment for the Isolation of Salmonella from Surface Water
Autumn L Kraft, Manan Sharma, James E Wells, Abasiofiok Mark Ibekwe, NARMS EWG, Jonathan G Frye
Published: 2022-06-15 DOI: 10.17504/protocols.io.ewov1n3xygr2/v1
National Program 108
Environmental Microbial and Food Safety Laboratory
Sureface water
Salmonella
water enrichment
Abstract
This protocol details the bulk water enrichment for the isolation of Salmonella from surface water.
Attachments
Steps
Protocol
1.
Take 1 of the water sample bottles (containing 1L of surface water) and carefully transfer water into a sterile 2 L Pyrex bottle.
2.
If needed, add control strain to water sample by gently tipping vial over the opening. Close bottle tightly and thoroughly mix sample.
3.
Aseptically measure 250mL of 5X UPB and pour into 2 L bottle (making final concentration of 1X UPB)
4. For next steps, refer to “Selective Enrichment Protocol for Salmonella Isolation from Surface Water.”
Secure lid and mix thoroughly before incubating at 37°C for 18-24 h.
Note
