Primer TE (10mM Tris, 0.1mM EDTA, pH8.0)
Jacquelina.Woods
Published: 2021-12-02 DOI: 10.17504/protocols.io.byccpssw
Abstract
This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR detection, library prep, genome sequencing, quality control checks, and data submission to NCBI. This method provides a reagent formula required in the extraction of RNA from viral concentrates using the RNeasy and Zymo kits.
Steps
1.
Mix components together.
1.1.
100µL
1.2.
20µL
1.3.
9.88mL
2.
Store at Room temperature.
