Preparing Cholesterol for use in NGM
Bonnie Evans
Published: 2023-10-17 DOI: 10.17504/protocols.io.36wgq3oe5lk5/v1
Abstract
C. elegans requires cholesterol to progress through the four larval stages.
Steps
1.
Prepare 5 mg/ml in Ethanol by resuspending the whole 250mg vial in 50 ml ~100% ethanol.
2.
Filter sterilise using vacuum pump.
3.
Store at 4 degrees protected from the light
