Preparation of M9 worm buffer

Alfonso Pérez Escudero, Alid Al-Asmar, Gabriel Madirolas

Published: 2022-07-20 DOI: 10.17504/protocols.io.x54v9yo6pg3e/v2
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Abstract

Preparation of M9 worm buffer, used to maintain Caenorhabditis elegans in liquid for short periods of time. Protocol taken from the WormBook Methods http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html

Steps

1.

Add 3g of monopotassium phosphate (KH2PO4) in a 1L clean bottle.

2.

Add 7.52g of disodium phosphate dihydrate (Na2HPO4.2H2O).

3.

Add 5g of sodium chloride (NaCl).

4.

Add 1L of milliQ water.

5.

Autoclave.

6.

Add 1mL of magnesium sulfate solution. Our protocol for making this solution can be found here:

Preparation of 1M magnesium sulfate solution (MgSO4)

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