Preparation of BG-11 liquid media
Ashwinuday
Published: 2021-10-18 DOI: 10.17504/protocols.io.by6qpzdw
Abstract
This is for making liquid media of BG-11.
Steps
1.
Take the medium in powdered form and mix it with doubly distilled water to just 1/10 th of the total volume.
2.
Add a common stock of micronutrients made in ddH2O and mix well.
3.
Make up the volume to the total and mixed well.
4.
Adjust the pH on a calibrated pH meter using 1N HCl and /or 1N NaOH. The media was mixed using a magnetic stirrer continuously to homogenize the pH and to prevent precipitation of certain constituents.
5.
This mix was autoclaved.
