Preparation and Administration of Systemic 4-AP
Leonardo A Parra-Rivas
Published: 2023-11-12 DOI: 10.17504/protocols.io.5qpvo38dxv4o/v1
Abstract
Preparation and Administration of Systemic 4-AP
Steps
1.
4-Aminopyridine was obtained from Sigma-Aldrich (Cat #A78403), and a stock solution was
prepared in saline. Mice (8 weeks old) were given intraperitoneal injections of
soluble 4-AP at a low dose of 2 mg/Kg or equivalent saline.
2.
Animals were monitored every 20 min and presented no sign of convulsive seizure behavior.
After 3 hrs, animals were anesthetized with isoflurane (VetOne Cat#502017),
followed by cervical dislocation. Brains were rapidly removed and drop-fixed in
4% PFA solution for immunohistochemistry or placed on ice for biochemical
assays.
