PROTEIN INJECTION PROTOCOL
Scott Vermilyea
Published: 2024-03-21 DOI: 10.17504/protocols.io.dm6gp3738vzp/v1
Abstract
This protocol details the injection parameters of the protein.
Attachments
Steps
Injection Parameters
1.
Stereotaxic coordinates:
1.1.
Intracortical/Intrastriatal (IC/IS): 2.0 mm lateral from the midline, +0.2 mm relative to bregma, and 0.8 and 2.6 mm deep from the dura.
1.2.
Brainstem: 0.2 mm lateral from the midline, -7.34 mm from bregma, 3.75 mm deep from the dura.
2.
Injectable:
2.1.
2.5µg of total protein in a volume of 2.5µL.
2.2.
Rate: 0.1µL per minute
2.3.
Needle: 28 ga needle attached via tubing to a Hamilton syringe controlled by a constant pressure syringe pump (Harvard Apparatus, Holliston, MA).
