Overview of scRNA-seq of Human Knee Articular Cartilage
molmer , Martin Lotz
Published: 2022-04-30 DOI: 10.17504/protocols.io.5qpvob93dl4o/v1
Abstract
The overview provides sub-protocols for each step required to complete scRNA-seq from human knee articular cartilage.
Steps
1.
Tissue Acquisition
2.
Tissue Quality Control
3.
Single-Cell Isolation
4.
cDNA Library Preparation
5.
Data Processing and Quality Control
