Overall protocol for top-down LC-MS/MS of human small intestine tissue

Jeannie Camarillo, Bryon Drown, Neil Kelleher

Published: 2022-03-02 DOI: 10.17504/protocols.io.b5tbq6in
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Abstract

Overview description of the process for acquiring top-down LC-MS/MS data on human small intestine tissue

Steps

Tissue Collection

1.

Small intestine tissue was provided by Stanford University TMC. Tissue was collected according to the following:

https://dx.doi.org/10.17504/protocols.io.bin8kdhw

Sample Preparation

2.

Perform LC based Proteomics on adjacent tissue sections:

Tissue Homogenization and GELFrEE for Top-Down Proteomics

Data Acquisition

3.

Untargeted Top-down Proteomics by LC-MS/MS on Lumos

4.

Top-down proteomics data analysis on the NRTDP TDPortal

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