Overall protocol for top-down CZE-MS/MS of human heart tissue

Jeannie Camarillo, Bryon Drown, Neil Kelleher

Published: 2022-03-04 DOI: 10.17504/protocols.io.b5seq6be

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Abstract

Overview description of the process for acquiring top-down CZE-MS/MS data on human heart tissue

Steps

Tissue Collection

1.

Heart tissue was provided by University of Washington. Tissue was collected and prepared according to the following:

Protocol for Tissue Collection from Organ Procurement Organization

Sample Preparation

2.

Perform LC based Proteomics on adjacent tissue sections:

Tissue Homogenization and GELFrEE for Top-Down Proteomics

Data Acquisition

3.

Untargeted Top-down Proteomics by CZE-MS/MS on Eclipse

4.

Top-down proteomics data analysis on the NRTDP TDPortal

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