Optimized single-nucleus transcriptional profiling by combinatorial indexing

Beth K. Martin, Chengxiang Qiu, Jay Shendure, Eva Nichols, Melissa Phung, Rula Green-Gladden, Sanjay Srivatsan, Ronnie Blecher-Gonen, Brian J. Beliveau, Cole Trapnell, Junyue Cao

Published: 2022-10-18 DOI: 10.1038/s41596-022-00752-0

optimization

single-nucleus transcriptional profiling

combinatorial indexing

sci-RNA-seq

whole-organism analysis

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Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) is a powerful method for recovering gene expression data from an exponentially scalable number of individual cells or nuclei. However, sci-RNA-seq is a complex protocol that has historically
Introduction
Materials
Procedure
Troubleshooting
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- Anticipated results
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References
ACKNOWLEDGMENTS

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Optimized single-nucleus transcriptional profiling by combinatorial indexing

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