Optical densitometry of neuronal fibers

Miquel Vila

Published: 2023-10-19 DOI: 10.17504/protocols.io.8epv5x3nng1b/v1

浏览 1059

Abstract

Optical densitometry of striatal neuronal fibers in the rodent brain

Steps

1.

Scan the immunostained serial coronal sections covering different caudorostral levels of the brain regions (for striatum, 4 sections/animal) with an Epson Perfection v750 Pro scanner.

2.

Open the resulting images in ImageJ (RRID:SCR_003070,https://imagej.net/).

3.

With the free hand selections tool, draw the region of interest (e.g. striatum, nucleus accumbens and olfactory tubercle) and measure the optical density (pixel brightness). Then draw a region of interest in a blank area (e.g. cortex) and also measure the optical density.

4.

Calculate the optical density or absorbance using the formula: -log10 (Striatum Intensity/Cortex Intensity).

推荐阅读

Nature Protocols

Protocols IO

Current Protocols

Fabrication of angstrom-scale two-dimensional channels for mass transport

Efficient and strand-specific profiling of replicating chromatin with enrichment and sequencing of protein-associated nascent DNA in mammalian cells

The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts

Rapid reaction optimization by robust and economical quantitative benchtop19F NMR spectroscopy

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Photoimmunotechnology as a powerful biological tool for molecular-based elimination of target cells and microbes, including bacteria, fungi and viruses

Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Preparation, validation and use of a vasoactive tryptophan-derived hydroperoxide and relevant control compounds

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes

查看全部

扫码咨询