Odor Threshold
Bryan Killinger, sdwalto, Jeffrey Kordower
Abstract
Odor threshold optimized for mice. This test was made to test short term olfactory memory. Mice with olfactory impairment or deficits should score lower.
Steps
Prepare and empty rodent cage with no bedding.
Prepare the odorant by diluting in mineral oil. (See guidelines)
Acclimate mouse to setup (empty cage with odor cartridges). Place mouse in the cage and expose to empty odor cartridge for 5 mins.
Pre-habituate mouse to a paper swab soaked in mineral oil in the odor cartridge. (5 mins).
Habituation phase . Expose mouse to mineral oil for three 50 secs trials with a 5 min interval between trials.
Detection phase . Expose mouse to lowest concentration of propionic acid for 50 secs and record time spent investigating the odor cartridge.
Repeat step 6 with the other concentrations of propionic acid, starting from lowest to highest.
