OCT Embedding
Joanna Bi
Published: 2024-01-19 DOI: 10.17504/protocols.io.3byl4qy6ovo5/v1
Abstract
OCT Embedding protocol
Steps
1.
Sample tissue is collected fresh.
2.
Squeeze a thin layer of OCT compound (Sakura Tissue-Tek O.C.T. Compound, ref 4583) into a disposable mold.
3.
Place the sample tissue in the center of the mold, with the side to be cut oriented into the mold.
4.
Squeeze more OCT compound over the top to full cover the tissue.
5.
Lower the mold into liquid nitrogen, being careful to not fully submerge the mold. Hold in liquid nitrogen for a few seconds until fully frozen.
6.
Place frozen mold in dry ice until stored in -80ºC
