Neural activation of the GI tract
Bryan Yoo, Jessica Griffiths, Sarkis Mazmanian
Abstract
Protocol for neural activation of the GI tract used in Yoo et al 2021
Steps
TH-Cre and ChAT-Cre mice are used for these experiments.
“Activated” mice are infected with AAV-PHP.S:hSYN1-DIO-hM3Dq-mRuby2 and “Control Mice” are infected with AAV-PHP.S:hSYN1-DIO-mRuby2. This is to control for both AAV-PHP.S-mediated expression and the effects of Compound 21 dihydrochloride (C21) (HelloBio, Princeton, NJ-HB6124).
C21 was injected intraperitoneally at a dose of 3mg/kg in both activated and control mice. Mice for time course experiments were single-housed in sterile cages with autoclaved water following first C21 administration (C21 administered for 10 consecutive days). Injections of C21 we
Mice for time course experiments were single-housed in sterile cages with autoclaved water following first C21 administration (C21 administered for 10 consecutive days).Injections of C21 were administered at the same time every day (10AM).
