NCEM Drop - Inactivation of sample in solution (TM-014)
sandra.crameri
Published: 2023-05-13 DOI: 10.17504/protocols.io.5jyl89k57v2w/v2
Abstract
This inactivation procedure is used to inactivate a sample for Biological Transfer out of PC4, proir to Conventional NCEM.
Steps
Inactivation
1.
Add 50µL 16% volume
2.
Add 150µL sample to the 0h 10m 0s
3.
Adsorb 10µL sample to grid 0h 10m 0s , inspect to ensure sample doesn't dry out.
4.
Drain excess sample from grid using fliter paper, leave wet.
5.
Stain 0h 1m 0s
6.
Drain & dry using filter paper
