Multimodal imaging pipeline for molecular and cellular characterization of Liver tissue - HuBMAP TTD-Columbia/PSU
Aubrianna Decker, Presha Rajbhandari, Brent R Stockwell, Hua Tian, Taruna Neelakantan
Abstract
We have developed a workflow to assess the spatial organization of liver tissue with regard to its cellular and metabolic heterogeneity at tissue and single cell level resolution - by performing multimodal imaging on consecutive tissue sections using H&E staining, DESI, (H2O)n-GCIB-SIMS, C60-SIMS, and RNAscope.
This document provides the collection of protocols within the multimodal imaging workflow implemented by Columbia University/Pennsylvania State University Transformative Technology Development Center as a part of Human Biomolecular Atlas Program (HuBMAP, NIH).
Steps
Acquire human tissue blocks.
Prepare tissue sections for multimodal imaging on appropriate substrates. dx.doi.org/10.17504/protocols.io.e6nvwjmjzlmk/v11
H&E staining is performed using standard protocol.
DESI-IMS is performed as described here.
(H2O)n-GCIB-SIMS-IMS is performed as described here.
C60-SIMS for multiplexed antibody staining is performed as described here.
RNAScope imaging is performed as described here.