Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags

Piotr Michaluk, Dmitri A. Rusakov

Published: 2022-09-04 DOI: 10.1038/s41596-022-00732-4

pH-sensitive fluorescent tag

cell membrane recycling

fluorescence recovery after photobleaching

GLT1 transporter

live-cell imaging

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Population behavior of signaling molecules on the cell surface is key to their adaptive function. Live imaging of proteins tagged with fluorescent molecules has been an essential tool in understanding this behavior. Typically, genetic or chemical tags are
Introduction
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ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags

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