Membrane Tube Image Analysis
Liv Jensen
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Abstract
This protocol details Membrane Tube Image Analysis.
Attachments
Steps
Membrane Tube Image Analysis
In FIJI, create new image stacks from two rectangular selections: one containing a section of the membrane tube, and one containing the approximately horizontal section of the guv.
Save these new stacks as .tif files and paste paths into enrichment quantification script.
Run enrichment quantification, adjusting parameters such as ktub to fit your experimental conditions.
