Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

Elizabeth Barber, Michael J. Houghton, Rizliya Visvanathan, Gary Williamson

Published: 2022-09-29 DOI: 10.1038/s41596-022-00736-0

HPAE-PAD

carbohydrate digestion

alpha-amylase

alpha-glucosidase

enzyme activity

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Carbohydrate digestion in the mammalian gastrointestinal tract is catalyzed by α-amylases and α-glucosidases to produce monosaccharides for absorption. Inhibition of these enzymes is the major activity of the drugs acarbose and miglitol, w
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Materials
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References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection

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