Large-scale growth ofC. elegansand isolation of membrane protein complexes

Eric Gouaux, Sarah Clark, Hanbin Jeong, April Goehring, Yunsik Kang

Published: 2023-07-25 DOI: 10.1038/s41596-023-00852-5

Extended

Extended Data Fig. 1 Comparison of sonication and cryo-bead milling methods for lysis of C. elegans .

Representative FSEC trace of affinity purified TMC-2-mVenus isolated from worms that were either lysed with cryo-bead milling (black traces) or sonication (blue traces). Data from three separate worm harvests are shown to illustrate that cryo-bead milling reproducibly results in a higher ratio of TMC-2 dimer to monomer.

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Large-scale growth ofC. elegansand isolation of membrane protein complexes

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