Interrogating the configurational stability of atropisomers Jean-Paul Heeb, Jonathan Clayden, Martin D. Smith, Roly J. Armstrong Published: 2023-08-03 DOI: 10.1038/s41596-023-00859-y atropisomers configurational stability racemization rate HPLC NMR AI 解读 浏览 1402 PDFMetadataSupplementary information Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols High-throughput single-cell transcriptomics of bacteria using combinatorial barcoding Inducible mouse models of colon cancer for the analysis of sporadic and inflammation-driven tumor progression and lymph node metastasis An unbiased method for evaluating the genome-wide specificity of base editors in rice Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH Designing and executing prime editing experiments in mammalian cells Design and application of the transformer base editor in mammalian cells and mice Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting Routine culture and study of adult human brain cells from neurosurgical specimens Simultaneously measuring the methylation of parent and daughter strands of replicated DNA at the single-molecule level by Hammer-seq Spatial visualization of comprehensive brain neurotransmitter systems and neuroactive substances by selective in situ chemical derivatization mass spectrometry imaging 查看全部 Mapping CGRP-IR innervation of male mice stomach with Neurolucida 360 CHOP TMC Single Cell Multiome ATAC + Gene Expression HuBMAP Children's Hospital of Philadelphia TMC Cloning individual AAV capsid variants ASAPCRN Cell culture Targeted ExSeq -- In Situ Sequencing (Illumina Chemistry) expansion microscopy in situ sequencing expansion sequencing targeted ExSeq ExSeq spatial transcriptomics spatial omics spatially resolved transcriptomics Well-tempered Metadynamics protocol Synaptic immunohistochemistry - wholemount via acetone permeabilization Recombinant αS purification Protocol ASAPCRN protocols.io academic and non-profit contract Panicle Ratio Network: A high-throughput dynamic phenotype recognition model based on ultra-high-definition unmanned aerial vehicle images for rice panicle analysis in fields 查看全部 Prediction of Acute Hepatotoxicity With Human Pluripotent Stem Cell-Derived Hepatic Organoids cytochrome P450 drug testing hepatic organoid hepatotoxicity human pluripotent stem cell differentiation liver A Step-by-Step Guide for the Production of Recombinant Fluorescent TAT-HA-Tagged Proteins and their Transduction into Mammalian Cells DNA molecular assembly fluorescent protein fusion protein Ni-NTA-based protein purification protein transduction recombinant protein production tagged protein MHC II–EGFP Knock-in Mouse Model APCs light-sheet fluorescence microscopy lymphoid organs MHC II–EGFP knock-in mouse quantitative immunology SARS-CoV-2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup COVID19 COVID-19 ELISA protein expression SARS-CoV-2 serological assay Generation and Functional Characterization of Monocytes and Macrophages Derived from Human Induced Pluripotent Stem Cells differentiation functional characterization induced pluripotent stem cells macrophages monocytes Super-Resolution Stimulated Raman Scattering Microscopy with Graphical User Interface–Supported A-PoD deconvolution metabolic imaging stimulated Raman scattering super-resolution Protein Structural Modeling for Electron Microscopy Maps Using VESPER and MAINMAST cryo-EM alignment de novo protein modeling electron microscopy maps protein fitting protein structure Hepatitis E Virus: Isolation, Propagation, and Quantification HEV isolation propagation virus quantification Do more with Less: Improving High Parameter Cytometry Through Overnight Staining antibody incubation high-parameter cytometry optimization overnight staining spectral cytometry titration Isolation and Culturing of Primary Murine Chondroprogenitor Cells: A Mammalian Model of Chondrogenesis cartilage formation chondrogenesis limb bud micromass culture mouse embryo 查看全部