In vivo fluorescent labeling and tracking of extracellular matrix

Adrian Fischer, Donovan Correa-Gallegos, Juliane Wannemacher, Simon Christ, Hans-Günther Machens, Yuval Rinkevich

Published: 2023-08-08 DOI: 10.1038/s41596-023-00867-y

fluorescent labeling

extracellular matrix

N-hydroxysuccinimide esters

tissue dynamics

organ development

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Sections

Figures

References

Introduction
Materials
Reagent setup
Equipment setup
- Surgical material and Whatman paper confetti
Procedure 1: subcutaneous fascia ECM labeling for full-thickness skin wound healing studies
Procedure 2: abdominal organ surface ECM labeling
- Laparotomy and organ surface ECM labeling
- Whole-mount preparation
Troubleshooting
Timing
- Procedure 1: widespread subcutaneous fascia labeling
- Procedure 2: area-specific organ surface labeling
Anticipated results
- Fascia ECM labeling in skin wound healing
- Organ surface labeling of ECM
Acknowledgements

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In vivo fluorescent labeling and tracking of extracellular matrix

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