High-throughput identification and quantification of bacterial cells in the microbiota based on 16S rRNA sequencing with single-base accuracy using BarBIQ

Jianshi Jin, Reiko Yamamoto, Katsuyuki Shiroguchi

Published: 2023-11-27 DOI: 10.1038/s41596-023-00906-8

Barcoding

Microbiota

16S rRNA Sequencing

Single-Base Accuracy

Bacterial Quantification

AI 解读

Supplementary information

Supplementary Information

Supplementary Methods 1 and 2.

Reporting Summary

Supplementary Table 1

Information of oligomers and barcodes.

Supplementary Table 2

16S rRNA sequences of bacteria in mock community.

Supplementary Table 3

Quantification of bacteria in mock community.

Supplementary Table 4

16S rRNA sequences of bacteria in cecal samples.

Supplementary Table 5

Quantification of bacteria in cecal samples.

Supplementary Data 1

Example of ‘sample sheet’ for a MiSeq run.

Supplementary Data 2

16S rRNA sequences of cecal samples in fasta format with predicted taxonomies.

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

Identification of cancer-related mutations in human pluripotent stem cells using RNA-seq analysis

Spiral volumetric optoacoustic tomography for imaging whole-body biodynamics in small animals

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

Tutorial: design and execution of CRISPR in vivo screens

Reproducible strategy for excisional skin-wound-healing studies in mice

CRISPR–Cas9-mediated chromosome engineering inArabidopsis thaliana

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging

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References

Supplementary information

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High-throughput identification and quantification of bacterial cells in the microbiota based on 16S rRNA sequencing with single-base accuracy using BarBIQ

Supplementary information

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