Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

Shih-Ching Lin, Kei Haga, Xi-Lei Zeng, Mary K. Estes

Published: 2022-02-22 DOI: 10.1038/s41596-021-00669-0

CRISPR-Cas9

genetic knockout

intestinal organoids

lentivirus transduction

single-cell cloning

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Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

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