FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing

Yanping Long, Jinbu Jia, Weipeng Mo, Xianhao Jin, Jixian Zhai

Published: 2021-07-29 DOI: 10.1038/s41596-021-00581-7

FLEP-seq

nascent RNA sequencing

cotranscriptional splicing

polyadenylation

kinetics

AI 解读

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Elongation, splicing and polyadenylation are fundamental steps of transcription, and studying their coordination requires simultaneous monitoring of these dynamic processes on one transcript. We recently developed a full-length nascent RNA sequencing meth
Introduction
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Anticipated results
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ACKNOWLEDGMENTS

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FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing

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