Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts

Xiaodong Liu, Jia Ping Tan, Jose M. Polo

Published: 2022-10-13 DOI: 10.1038/s41596-022-00742-2

induced trophoblast stem cells

fibroblast reprogramming

Yamanaka factors

patient-specific iTS cells

trophoblast biology

AI 解读

Supplementary information

Supplementary Information

Flow cytometry gating strategy for live cells

Author Correction: Full-length sequencing of circular DNA viruses and extrachromosomal circular DNA using CIDER-Seq

Generation of heart-forming organoids from human pluripotent stem cells

Design and fabrication of wearable electronic textiles using twisted fiber-based threads

Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo

Chromatin accessibility profiling by ATAC-seq

Deoxyfluorination of phenols for chemoselective18F-labeling of peptides

Author Correction: cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

An inexpensive semi-automated sample processing pipeline for cell-free RNA extraction

Application of CHyMErA Cas9-Cas12a combinatorial genome-editing platform for genetic interaction mapping and gene fragment deletion screening

查看全部

Sections

Figures

References

Supplementary information

Thomson, J. A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147 (1998).
Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861–872 (2007).
Yu, J. et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917–1920 (2007).
Orendi, K. et al. Placental and trophoblastic in vitro models to study preventive and therapeutic agents for preeclampsia. Placenta 32 (Suppl.), S49–S54 (2011).
Sooranna, S. R., Oteng-Ntim, E., Meah, R., Ryder, T. A. & Bajoria, R. Characterization of human placental explants: morphological, biochemical and physiological studies using first and third trimester placenta. Hum. Reprod. 14, 536–541 (1999).
Turco, M. Y. & Moffett, A. Development of the human placenta. Development 146, dev163428 (2019).
Heazlewood, C. F. et al. High incidence of contaminating maternal cell overgrowth in human placental mesenchymal stem/stromal cell cultures: a systematic review. Stem Cells Transl. Med. 3, 1305–1311 (2014).
Apps, R. et al. Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells. Placenta 32, 33–43 (2011).
Pattillo, R. A. & Gey, G. O. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 28, 1231–1236 (1968).
Kohler, P. O. & Bridson, W. E. Isolation of hormone-producing clonal lines of human choriocarcinoma. J. Clin. Endocrinol. Metab. 32, 683–687 (1971).
Hertz, R. Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc. Soc. Exp. Biol. Med. 102, 77–81 (1959).
Amita, M. et al. Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4. Proc. Natl Acad. Sci. Us. A 110, E1212–E1221 (2013).
Xu, R.-H. et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat. Biotechnol. 20, 1261–1264 (2002).
Okae, H. et al. Derivation of human trophoblast stem cells. Cell Stem Cell 22, 50–63.e6 (2018).
Liu, X. et al. Reprogramming roadmap reveals route to human induced trophoblast stem cells. Nature 586, 101–107 (2020).
Mischler, A., Karakis, V., Mahinthakumar, J. & Carberry, C. Two distinct trophectoderm lineage stem cells from human pluripotent stem cells. J. Biol. Chem. 296, 100386 (2021).
Wei, Y. et al. Efficient derivation of human trophoblast stem cells from primed pluripotent stem cells. Sci Adv 7, (2021).
Li, Z., Kurosawa, O. & Iwata, H. Establishment of human trophoblast stem cells from human induced pluripotent stem cell-derived cystic cells under micromesh culture. Stem Cell Res. Ther. 10, 245 (2019).
Dong, C. et al. Derivation of trophoblast stem cells from naïve human pluripotent stem cells. eLife 9, e52504 (2020).
Cinkornpumin, J. K. et al. Naive human embryonic stem cells can give rise to cells with a trophoblast-like transcriptome and methylome. Stem Cell Rep. 15, 198–213 (2020).
Guo, G. et al. Human naive epiblast cells possess unrestricted lineage potential. Cell. Stem Cell. 28, 1040–1056.e6 (2021).
Io, S. et al. Capturing human trophoblast development with naive pluripotent stem cells in vitro. Cell Stem Cell 28, 1023–1039.e13 (2021).
Castel, G. et al. Induction of human trophoblast stem cells from somatic cells and pluripotent stem cells. Cell Rep. 33, 108419 (2020).
Bayerl, J. et al. Principles of signaling pathway modulation for enhancing human naive pluripotency induction. Cell Stem Cell 28, 1549–1565.e12 (2021).
Castel, G. & David, L. Induction of human trophoblast stem cells. Nat. Protoc. https://doi.org/10.1038/s41596-022-00744-0 (2022).
Chang, C.-W., Wakeland, A. K. & Parast, M. M. Trophoblast lineage specification, differentiation and their regulation by oxygen tension. J. Endocrinol. 236, R43–R56 (2018).
Scifres, C. M. & Nelson, D. M. Intrauterine growth restriction, human placental development and trophoblast cell death. J. Physiol. 587, 3453–3458 (2009).
Sheridan, M. A. et al. Early onset preeclampsia in a model for human placental trophoblast. Proc. Natl Acad. Sci. USA 116, 4336–4345 (2019).
Alici-Garipcan, A., Özçimen, B., Suder, I. & Ülker, V. NLRP7 plays a functional role in regulating BMP4 signaling during differentiation of patient-derived trophoblasts. bioRxiv (2019).
Horii, M. et al. Human pluripotent stem cells as a model of trophoblast differentiation in both normal development and disease. Proc. Natl Acad. Sci. USA 113, E3882–E3891 (2016).
Takahashi, S. et al. Loss of p57KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells. Proc. Natl Acad. Sci. USA https://doi.org/10.1073/pnas.1916019116 (2019).
Yu, J. et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science 324, 797–801 (2009).
Warren, L. et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7, 618–630 (2010).
Turco, M. Y. et al. Trophoblast organoids as a model for maternal-fetal interactions during human placentation. Nature 564, 263–267 (2018).
Haider, S. et al. Self-renewing trophoblast organoids recapitulate the developmental program of the early human placenta. Stem Cell Rep. 11, 537–551 (2018).
Paynter, J. M., Chen, J., Liu, X. & Nefzger, C. M. Propagation and maintenance of mouse embryonic stem cells. Methods Mol. Biol. 1940, 33–45 (2019).
Liu, X. et al. Generation of mouse-induced pluripotent stem cells by lentiviral transduction. Methods Mol. Biol. 1940, 63–76 (2019).
CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit. https://www.thermofisher.com/order/catalog/product/A16517 (2022).
Muir, A., Lever, A. M. L. & Moffett, A. Human endogenous retrovirus-W envelope (syncytin) is expressed in both villous and extravillous trophoblast populations. J. Gen. Virol. 87, 2067–2071 (2006).
Lee, C. Q. E. et al. What is trophoblast? A combination of criteria define human first-trimester trophoblast. Stem Cell Rep. 6, 257–272 (2016).

Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts

Supplementary information

推荐阅读