Enzyme immobilization on covalent organic framework supports

Qianqian Zhu, Yunlong Zheng, Zhenjie Zhang, Yao Chen

Published: 2023-09-05 DOI: 10.1038/s41596-023-00868-x

Enzyme immobilization

Metal-organic frameworks

Covalent organic frameworks

Direct-immobilization

Biocomposites

AI 解读

The GFP thermal shift assay for screening ligand and lipid interactions to solute carrier transporters

Co-fractionation–mass spectrometry to characterize native mitochondrial protein assemblies in mammalian neurons and brain

Simultaneously measuring the methylation of parent and daughter strands of replicated DNA at the single-molecule level by Hammer-seq

The Nodewalk assay to quantitate chromatin fiber interactomes in very small cell populations

Nondestructive production of exosomes loaded with ultrathin palladium nanosheets for targeted bio-orthogonal catalysis

An optimized culture system for efficient derivation of porcine expanded potential stem cells from preimplantation embryos and by reprogramming somatic cells

Engineering SARS-CoV-2 using a reverse genetic system

cfSNV: a software tool for the sensitive detection of somatic mutations from cell-free DNA

Growing and making nano- and microcrystals

Composable microfluidic spinning platforms for facile production of biomimetic perfusable hydrogel microtubes

查看全部

Sections

Figures

References

Introduction
Materials
Procedure 1: synthesis of enzyme@COF capsules by using MOFs as protectors
Procedure 2: property characterization of enzyme@COF capsules by using MOFs as protectors
Procedure 3: synthesis enzyme@COF constructed via in situ direct-immobilization methods
Procedure 4: property characterization of enzyme@COF via in situ direct-immobilization methods
Procedure 5: general procedure for characterization of the samples
Troubleshooting
Timing
Anticipated results
Acknowledgements

Devine, P. N. et al. Extending the application of biocatalysis to meet the challenges of drug development. Nat. Rev. Chem. 2, 409–421 (2018).
Intasian, P. et al. Enzymes, in vivo biocatalysis, and metabolic engineering for enabling a circular economy and sustainability. Chem. Rev. 121, 10367–10451 (2021).
Sheldon, R. A. & Woodley, J. M. Role of biocatalysis in sustainable chemistry. Chem. Rev. 118, 801–838 (2018).
Sheldon, R. A. & van Pelt, S. Enzyme immobilisation in biocatalysis: why, what and how. Chem. Soc. Rev. 42, 6223–6235 (2013).
Liang, W. et al. Metal-organic framework-based enzyme biocomposites. Chem. Rev. 121, 1077–1129 (2021).
Cantone, S. et al. Efficient immobilisation of industrial biocatalysts: criteria and constraints for the selection of organic polymeric carriers and immobilisation methods. Chem. Soc. Rev. 42, 6262–6276 (2013).
Brogan, A. P. S., Bui-Le, L. & Hallett, J. P. Non-aqueous homogenous biocatalytic conversion of polysaccharides in ionic liquids using chemically modified glucosidase. Nat. Chem. 10, 859–865 (2018).
Quin, M. B. & Schmidt-Dannert, C. Engineering of biocatalysts: from evolution to creation. ACS Catal. 1, 1017–1021 (2011).
Sharma, A., Gupta, G., Ahmad, T., Mansoor, S. & Kaur, B. Enzyme engineering: current trends and future perspectives. Food Rev. Int. 37, 121–154 (2019).
Huang, S., Chen, G. & Ouyang, G. Confining enzymes in porous organic frameworks: from synthetic strategy and characterization to healthcare applications. Chem. Soc. Rev. 51, 6824–6863 (2022).
Mohamad, N. R., Marzuki, N. H., Buang, N. A., Huyop, F. & Wahab, R. A. An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes. Biotechnol. Biotechnol. Equip. 29, 205–220 (2015).
An, H. et al. Incorporation of biomolecules in metal-organic frameworks for advanced applications. Coord. Chem. Rev. 384, 90–106 (2019).
Hudson, S., Cooney, J. & Magner, E. Proteins in mesoporous silicates. Angew. Chem. Int. Ed. Engl. 47, 8582–8594 (2008).
Fried, D. I., Brieler, F. J. & Fröba, M. Designing inorganic porous materials for enzyme adsorption and applications in biocatalysis. ChemCatChem 5, 862–884 (2013).
Kandambeth, S. et al. Self-templated chemically stable hollow spherical covalent organic framework. Nat. Commun. 6, 6786 (2015).
Sun, Q., Aguila, B., Lan, P. C. & Ma, S. Tuning pore heterogeneity in covalent organic frameworks for enhanced enzyme accessibility and resistance against denaturants. Adv. Mater. 31, e1900008 (2019).
Côté, A. P. et al. Porous, crystalline, covalent organic frameworks. Science 310, 1166–1170 (2005).
Esrafili, A., Wagner, A., Inamdar, S. & Acharya, A. P. Covalent organic frameworks for biomedical applications. Adv. Healthc. Mater. 10, e2002090 (2021).
Chen, Y. et al. Cell membrane-anchoring covalent organic framework nanosheets for single-laser-triggered synergistic tumor therapy. Chem. Commun. 57, 11685–11688 (2021).
Sun, Q. et al. Covalent organic framework based nanoagent for enhanced mild-temperature photothermal therapy. Biomater. Sci. 9, 7977–7983 (2021).
Zhang, P. et al. Melt polymerization synthesis of a class of robust self-shaped olefin-linked COF foams as high-efficiency separators. Sci. China Chem. 65, 1173–1184 (2022).
Mu, Z. et al. Covalent organic frameworks with record pore apertures. J. Am. Chem. Soc. 144, 5145–5154 (2022).
Xing, C. et al. Enhancing enzyme activity by the modulation of covalent interactions in the confined channels of covalent organic frameworks. Angew. Chem. Int. Ed. Engl. 61, e202201378 (2022).
Wang, C. & Liao, K. Recent advances in emerging metal- and covalent-organic frameworks for enzyme encapsulation. ACS Appl. Mater. Interfaces 13, 56752–56776 (2021).
Sun, Q. et al. Pore environment control and enhanced performance of enzymes infiltrated in covalent organic frameworks. J. Am. Chem. Soc. 140, 984–992 (2018).
Tang, Y. et al. Fabrication of hollow covalent-organic framework microspheres via emulsion-interfacial strategy to enhance laccase immobilization for tetracycline degradation. Chem. Eng. J. 421, 129743 (2021).
Zhao, Z. et al. Engineering olefin-linked covalent organic frameworks for photoenzymatic reduction of CO2. Angew. Chem. Int. Ed. Engl. 61, e202200261 (2022).
Jin, C. et al. Enzyme immobilization in porphyrinic covalent organic frameworks for photoenzymatic asymmetric catalysis. ACS Catal. 12, 8259–8268 (2022).
Zhang, S. et al. Covalent organic frameworks with chirality enriched by biomolecules for efficient chiral separation. Angew. Chem. Int. Ed. Engl. 57, 16754–16759 (2018).
Chao, H. et al. Template-free in situ encapsulation of enzymes in hollow covalent organic framework capsules for the electrochemical analysis of biomarkers. ACS Appl. Mater. Interfaces 14, 20641–20651 (2022).
Huang, N., Wang, P. & Jiang, D. Covalent organic frameworks: a materials platform for structural and functional designs. Nat. Rev. Mater. 1, 16068 (2016).
Zheng, Y. et al. Green and scalable fabrication of high-performance biocatalysts using covalent organic frameworks as enzyme carriers. Angew. Chem. Int. Ed. Engl. 61, e202208744 (2022).
Liang, K. et al. Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules. Nat. Commun. 6, 7240 (2015).
Feng, Y. et al. Antibodies@MOFs: an in vitro protective coating for preparation and storage of biopharmaceuticals. Adv. Mater. 31, e1805148 (2019).
Li, M. et al. Fabricating covalent organic framework capsules with commodious microenvironment for enzymes. J. Am. Chem. Soc. 142, 6675–6681 (2020).
Liang, H., Wang, L., Yang, Y., Song, Y. & Wang, L. A novel biosensor based on multienzyme microcapsules constructed from covalent-organic framework. Biosens. Bioelectron. 193, 113553 (2021).
Chen, G. et al. A convenient and versatile amino-acid-boosted biomimetic strategy for the nondestructive encapsulation of biomacromolecules within metal-organic frameworks. Angew. Chem. Int. Ed. Engl. 58, 1463–1467 (2019).
Wu, Z. et al. Covalent organic networks for in situ entrapment of enzymes with superior robustness and durability. Chem. Eng. J. 450, 138446 (2022).
Maddigan, N. K. et al. Protein surface functionalisation as a general strategy for facilitating biomimetic mineralisation of ZIF-8. Chem. Sci. 9, 4217–4223 (2018).
Chen, G. et al. Modulating the biofunctionality of metal-organic-framework-encapsulated enzymes through controllable embedding patterns. Angew. Chem. Int. Ed. Engl. 59, 2867–2874 (2020).

Enzyme immobilization on covalent organic framework supports

Supplementary information

推荐阅读