Encapsulating and stabilizing enzymes using hydrogen-bonded organic frameworks

Guosheng Chen, Siming Huang, Xiaomin Ma, Rongwei He, Gangfeng Ouyang

Published: 2023-05-16 DOI: 10.1038/s41596-023-00828-5

Hydrogen-bonded Organic Frameworks

Enzyme Encapsulation

Stability

Biotransformations

Biocatalytic Performance

AI 解读

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Enzymes are outstanding natural catalysts with exquisite 3D structures, initiating countless life-sustaining biotransformations in living systems. The flexible structure of an enzyme, however, is highly susceptible to non-physiological environments, which
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

Lian, X. et al. Enzyme–MOF (metal–organic framework) composites. Chem. Soc. Rev. 46, 3386–3401 (2017).
Thornberry, N. A. & Lazebink, Y. Caspases: enemies within. Science 281, 1312–1316 (1998).
Henzler-Wildman, K. & Kern, D. Dynamic personalities of proteins. Nature 450, 964–972 (2007).
Sheldon, R. A. & Woodley, J. M. Role of biocatalysis in sustainable chemistry. Chem. Rev. 118, 801–838 (2018).
Intasian, P. et al. Enzymes, in vivo biocatalysis, and metabolic engineering for enabling a circular economy and sustainability. Chem. Rev. 121, 10367–10451 (2021).
Devine, P. N. et al. Extending the application of biocatalysis to meet the challenges of drug development. Nat. Rev. Chem. 2, 409–421 (2018).
Schmid, A. et al. Industrial biocatalysis today and tomorrow. Nature 409, 258–268 (2001).
Wong, L. S., Khan, F. & Micklefield, J. Selective covalent protein immobilization: strategies and applications. Chem. Rev. 109, 4025–4053 (2009).
Sheldon, R. A., Basso, A. & Brady, D. New frontiers in enzyme immobilisation: robust biocatalysts for a circular bio-based economy. Chem. Soc. Rev. 50, 5850–5862 (2021).
Huang, S., Chen, G. & Ouyang, G. Confining enzymes in porous organic frameworks: from synthetic strategy and characterization to healthcare applications. Chem. Soc. Rev. 51, 6824–6863 (2022).
Liang, W. et al. Metal–organic framework-based enzyme biocomposites. Chem. Rev. 121, 1077–1129 (2021).
Wang, B., Lin, R.-B., Zhang, Z., Xiang, S. & Chen, B. Hydrogen-bonded organic frameworks as a tunable platform for functional materials. J. Am. Chem. Soc. 142, 14399–14416 (2020).
Li, P., Ryder, M. R. & Stoddart, J. F. Hydrogen-bonded organic frameworks: a rising class of porous molecular materials. Acc. Mater. Res. 1, 77–87 (2020).
Song, X. et al. Design rules of hydrogen-bonded organic frameworks with high chemical and thermal stabilities. J. Am. Chem. Soc. 144, 10663–10687 (2022).
Lin, R.-B. & Chen, B. Hydrogen-bonded organic frameworks: chemistry and functions. Chem 8, 2114–2135 (2022).
Liang, W. et al. Enzyme encapsulation in a porous hydrogen-bonded organic framework. J. Am. Chem. Soc. 141, 14298–14305 (2019).
Wied, P. et al. Combining a genetically engineered oxidase with hydrogen-bonded organic frameworks (HOFs) for highly efficient biocomposites. Angew. Chem. Int. Ed. 61, e202117345 (2022).
Chen, G. et al. Protein-directed, hydrogen-bonded biohybrid framework. Chem 7, 2722–2742 (2021).
Tang, Z. et al. A biocatalytic cascade in an ultrastable mesoporous hydrogen-bonded organic framework for point-of-care biosensing. Angew. Chem. Int. Ed. 60, 23608–23613 (2021).
Tang, J. et al. In-situ encapsulation of protein into nanoscale hydrogen-bonded organic frameworks for intracellular biocatalysis. Angew. Chem. Int. Ed. 60, 22315–22321 (2021).
Chen, G. et al. Hydrogen-bonded organic framework biomimetic entrapment allowing non-native biocatalytic activity in enzyme. Nat. Commun. 13, 4816 (2022).
Nelson, J. & Griffin, E. G. Adsorption of invertase. J. Am. Chem. Soc. 38, 1109–1115 (1916).
Hartmann, M. & Kostrov, X. Immobilization of enzymes on porous silicas—benefits and challenges. Chem. Soc. Rev. 42, 6277–6289 (2013).
Pierre, A. C. The sol-gel encapsulation of enzymes. Biocatal. Biotransformation 22, 145–170 (2004).
Furukawa, H., Cordova, K. E., O’Keeffe, M. & Yaghi, O. M. The chemistry and applications of metal-organic frameworks. Science 341, 1230444 (2013).
Cote, A. P. et al. Porous, crystalline, covalent organic frameworks. Science 310, 1166–1170 (2005).
Lykourinou, V. et al. Immobilization of MP-11 into a mesoporous metal–organic framework, MP-11@mesoMOF: a new platform for enzymatic catalysis. J. Am. Chem. Soc. 133, 10382–10385 (2011).
Li, P. et al. Nanosizing a metal–organic framework enzyme carrier for accelerating nerve agent hydrolysis. ACS Nano 10, 9174–9182 (2016).
Li, P. et al. Hierarchically engineered mesoporous metal-organic frameworks toward cell-free immobilized enzyme systems. Chem 4, 1022–1034 (2018).
Sun, Q. et al. Pore environment control and enhanced performance of enzymes infiltrated in covalent organic frameworks. J. Am. Chem. Soc. 140, 984–992 (2018).
Lyu, F., Zhang, Y., Zare, R. N., Ge, J. & Liu, Z. One-pot synthesis of protein-embedded metal−organic frameworks with enhanced biological activities. Nano Lett. 14, 5761–5765 (2014).
Liang, K. et al. Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules. Nat. Commun. 6, 7240 (2015).
Li, M. et al. Fabricating covalent organic framework capsules with commodious microenvironment for enzymes. J. Am. Chem. Soc. 142, 6675–6681 (2020).
Zheng, Y. et al. Green and scalable fabrication of high-performance biocatalysts using covalent organic frameworks as enzyme carriers. Angew. Chem. Int. Ed. 61, e202208744 (2022).
Gao, R. et al. Mechanochemistry-guided reticular assembly for stabilizing enzymes with covalent organic frameworks. Cell Rep. Phys. Sci. 3, 101153 (2022).
Hu, C. et al. Defect-induced activity enhancement of enzyme-encapsulated metal-organic frameworks revealed in microfluidic gradient mixing synthesis. Sci. Adv. 6, eaax5785 (2020).
Liang, W. et al. Enhanced activity of enzymes encapsulated in hydrophilic metal–organic frameworks. J. Am. Chem. Soc. 141, 2348–2355 (2019).
Tong, L. et al. Atomically unveiling the structure-activity relationship of biomacromolecule-metal-organic frameworks symbiotic crystal. Nat. Commun. 13, 951 (2022).
Wu, X. et al. Packaging and delivering enzymes by amorphous metal-organic frameworks. Nat. Commun. 10, 5165 (2019).
Feng, Y. et al. Defect engineering of enzyme-embedded metal-organic frameworks for smart cargo release. Chem. Eng. J. 439, 135736 (2022).
Wu, X. et al. A versatile competitive coordination strategy for tailoring bioactive zeolitic imidazolate framework composites. Small 17, e2007586 (2021).
Huang, W. et al. Photodynamic hydrogen-bonded biohybrid framework: a photobiocatalytic cascade nanoreactor for accelerating diabetic wound therapy. JACS Au 2, 2048–2058 (2022).
Ye, N. et al. Hydrogen-bonded biohybrid framework-derived highly specific nanozymes for biomarker sensing. Anal. Chem. 93, 13981–13989 (2021).
Wijesundara, Y. H. et al. Carrier gas triggered controlled biolistic delivery of DNA and protein therapeutics from metal–organic frameworks. Chem. Sci. 13, 13803–13814 (2022).
Chen, T.-T., Yi, J.-T., Zhao, Y.-Y. & Chu, X. Biomineralized metal-organic framework nanoparticles enable intracellular delivery and endo-lysosomal release of native active proteins. J. Am. Chem. Soc. 140, 9912–9920 (2018).
Maddigan, N. K. et al. Protein surface functionalisation as a general strategy for facilitating biomimetic mineralisation of ZIF-8. Chem. Sci. 9, 4217–4223 (2018).
Shen, B. et al. A single-molecule van der Waals compass. Nature 592, 541–544 (2021).
Lazić, I. et al. Single-particle cryo-EM structures from iDPC–STEM at near-atomic resolution. Nat. Methods 19, 1126–1136 (2022).
Gao, L. et al. Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat. Nanotechnol. 2, 577–583 (2007).
Wei, H. & Wang, E. Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes. Chem. Soc. Rev. 42, 6060–6093 (2013).

Encapsulating and stabilizing enzymes using hydrogen-bonded organic frameworks

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