E. coli growth trend measurement (culture with silver ion)

Disclaimer

DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.

Abstract

This protocol helps obtain growth curve of E. coli cultured with concentration gradient of silver ion.

Steps

IPTG induction

Grow Escherichia coli overnight and dilute the culture using LB to OD₆₀₀=0.4-0.6.

Add IPTG to cell culture (final concentration 1mM), and incubate the culture 3h at 171 rpm, 37℃ in orbital shaking incubator.

Sample addition to microplate wells

Cells cultures, LB broth and silver nitrate (150μM) are added into 96-well microplate. The recipe is as the following chart:

E. coli cultured with concentration gradient of silver ion.

Microplate reader measurement

Incubate the microplate in orbital incubator (37℃ 171 rpm) and measure OD₆₀₀ by microplate reader every two hours for two days.