E. coli Culture

Add 2mL (3mL also acceptable) of LB medium and the appropriate antibiotic that selects for the target strain into a 15 mL tube.

Kanamycin 50 mg/mL Stock Solution 

Place the 15mL tube in the ThermoMixer at 37°C, 300 rpm, for 0h 5m 0s .

Equipment

Value	Label
ThermoMixer® C	NAME
Eppendorf	BRAND
Catalog No. 2231000680	SKU

Gather cells.

Swab a single colony on the agar plate with a sterile 1µL inoculation loop.

OR Dip a sterile 10 µL innoculation loop in a preexisting culture tube.

Innoculate the 15 mL tube with the loop.

Place the 15 mL tube in the thermomixer for 16 hours at 37°C and 300 rpm.