Defining AD and PD risk genes Xianjun Dong Published: 2023-11-18 DOI: 10.17504/protocols.io.kxygx329zg8j/v1 ASAPCRN AI 解读 浏览 1151 Abstract This protocol describes the method of how we define AD and PD risk genes based on the publications. Attachments protocol.ADPDriskgenes.pdf Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Opto-juxtacellular interrogation of neural circuits in freely moving mice Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia Self-assembled ultraflexible probes for long-term neural recordings and neuromodulation Acute head-fixed recordings in awake mice with multiple Neuropixels probes Creating hierarchical pores in metal–organic frameworks via postsynthetic reactions SorTn-seq: a high-throughput functional genomics approach to discovering regulators of bacterial gene expression Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing Scalable tissue labeling and clearing of intact human organs Analyzing somatic mutations by single-cell whole-genome sequencing 查看全部 Characterization of the VKORC1 and CYP2C9 genotypes Vitamin K Epoxide Reductase Vitamin KO Reductase VKORC1 Cytochrome P-450 CYP2C9 Magnetic bead cleaning of PCR products with Cytiva Sera-Mag Select unc-80 LoF Mutant Drug Repurposing/Confirmation Screening Protocol: MHC class I and dengue hemorrhagic fever: a systematic review of HLA-A*24 and HLA-B*44 Creating Differential Transcript Expression Results with DESeq2 DESeq2 nanopore transcript DE expression Visualizing lower urinary tract afferent projections in the lumbosacral spinal cord in rats neuroscience retrograde tracing immunohistochemistry clearing iDISCO 3D reconstruction neuroanatomy First Strand Synthesis with Reverse Transcriptase (M0253) M-MuLV reverse transcriptase first strand reverse transcription transcription Community-based cross-sectional survey Liquid chromatography-mass spectrometry method for isomer separation and detection of sugars, phophorylated sugars and organic acids Central metabolism isomer separation isotopic labeling liquid chromatography-mass spectrometry metabolite quantification mixed-mode column chromatography Mouse Whole Cell Tissue Processing for 10x Genomics Platform 10x genomics whole cell transcriptomic isolate extraction processing preparation cell sorting cell suspension PF0307 查看全部 Using Mothur to Determine Bacterial Community Composition and Structure in 16S Ribosomal RNA Datasets 16S rRNA microbiome OTUs Validation of a Novel, Flash-Freezing Method: Aluminum Platform DNA fresh tissue immunofluorescence liver pancreas RNA Culturing and Mating of Aspergillus fumigatus ascospores Aspergillus fumigatus cleistothecia mating sexual reproduction Small Interfering RNA Delivery Into Primary Human Natural Killer Cells for Functional Gene Analyses cell therapy gene function knockdown NK cells siRNA An In Vivo Mouse Model for Chronic Inflammation–Induced Immune Suppression: A “Factory” for Myeloid-Derived Suppressor Cells (MDSCs) chronic inflammation immune suppression M-MDSCs myeloid cells PMN-MDSCs Purification of Adeno-Associated Viral Vector Serotype 9 Using Ceramic Hydroxyapatite Chromatography and its Analysis AAV ceramic hydroxyapatite chromatography purification TFF UniLectin, A One-Stop-Shop to Explore and Study Carbohydrate-Binding Proteins carbohydrate glycan-binding lectin structural biology Purification and Application of Genetically Encoded Potassium Ion Indicators for Quantification of Potassium Ion Concentrations within Biological Samples cell viability FRET GEPIIs K+ potassium ions recombinant protein serum K+ Mouse Models of Sepsis cecal slurry sepsis CLP microbial sepsis mouse models sepsis Three-Dimensional Vessels-on-a-Chip Based on hiPSC-derived Vascular Endothelial and Smooth Muscle Cells differentiation endothelial cells human induced pluripotent stem cells neural crest vascular smooth muscle cells vessels-on-a-chip 查看全部
