DNA extraction protocol for saliva samples obtained from Omigene oral OM device using the Qiagen QiaAMP Powerfecal DNA kit for shotgun metagenomic sequencing
Aymeric David, christian.morabito, Victoria Meslier, Mathieu Almeida
Published: 2024-02-13 DOI: 10.17504/protocols.io.eq2lyjyrplx9/v1
Abstract
This protocol describes the procedure for DNA extraction of saliva samples using the Omnigene OM device and the Qiagen QiaMP Powerfecal DNA PRO kit for shotgun metagenomic sequencing.
Before start
visit the protocols.io for saliva sampling : https://dx.doi.org/10.17504/protocols.io.n92ldm8qol5b/v1
Attachments
Steps
Salivary Collection Using OMNIgene ORAL OM 501 Device
1.
Visit the protocols.io for saliva sampling : https://dx.doi.org/10.17504/protocols.io.n92ldm8qol5b/v1
Sample pre-processing
2.
Heat the whole OMNIgene Oral OM 501 tubes into an incubator at 50 °C for 2 h.
DNA extraction QIAGEN QIAamp Power Fecal Pro
3.
Available at https://www.qiagen.com/
