DNA barcoding SOPs for the Darwin Tree of Life Project

Michelle Hart, Laura L Forrest, Rebekka Uhl, Jordan Beasley, Rebecca Yahr, Estelle Kilias, David Bell

Published: 2023-11-14 DOI: 10.17504/protocols.io.261ged91jv47/v1

Abstract

This collection of protocols describe the standard operating procedures for DNA barcoding in the Darwin Tree of Life project.

The SOPs cover barcoding of macroalgae and marine protists, fungi and lichens at the Marine Biological Association, protists at the University of Oxford, plants and lichens at the Royal Botanic Gardens of Edinburgh and animals at the Natural History Museum of London.

Steps

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