Combinatorial fluorescent labeling of live anaerobic bacteria via the incorporation of azide-modified sugars into newly synthesized macromolecules

Haitham Hajjo, Neerupma Bhardwaj, Tal Gefen, Naama Geva-Zatorsky

Published: 2023-10-10 DOI: 10.1038/s41596-023-00896-7

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Extended Data Fig. 1 Assessment of the nonspecific binding of cyclooctyne fluorophores using confocal microscopy.

To assess the nonspecific binding of cyclooctyne fluorophores to bacteria, B. fragilis was grown in basal media (BM) or GalNAz-supplemented basal media (GalNAz) and then incubated with AZDye647, AZDye488 and TAMRA cyclooctyne fluorophores. The fluorescence was assessed by confocal microscopy imaging. Gamma and range correction techniques were identically applied to both the images and their respective controls.

Extended Data Table 1 Bacterial strains that were successfully labeled with this protocol using GalNAz, ManNAz and GlcNAz AMSs

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Extended Data Table. 2 Bacterial strains that were not successfully labeled with this protocol (using GalNAz, ManNAz and GlcNAz AMSs

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Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

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Combinatorial fluorescent labeling of live anaerobic bacteria via the incorporation of azide-modified sugars into newly synthesized macromolecules

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