Cell viability assessment
Qing Wang
Published: 2023-04-07 DOI: 10.17504/protocols.io.eq2ly7rpelx9/v1
Abstract
This is the protocol for assessing cell viability using a MTS cell proliferation assay kit.
Steps
1.
- Cells are seeded and differentiated for 4 days; assess cell viability using an MTS cell proliferation assay kit (ab197010, Abcam).
2.
- Treat the differentiated cells with synthetic DOPA pheomelanin or synthetic DOPA eumelanin or vehicle PBS to determine the time and dose responses of the cells to the treatment.
3.
- Remove culture medium and add a 110 µL solution containing 10 µL MTS and 100 µL medium to each well, incubate in humidified air containing 5% CO2 at 37°C for 1 hour.
4.
- Measure absorbance at 490 nm using a microplate reader.
