Catalepsy test in rats

Veerle Baekelandt, María Sanchiz Calvo, Eduard Bentea

Published: 2024-02-13 DOI: 10.17504/protocols.io.q26g7p24kgwz/v1

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Abstract

Protocol for performing the catalepsy bar test in rats. This test evaluates muscular rigidity, and can be used in rat models with motor deficits, such as models of Parkison's disease.

Steps

Test

1.

Bring cages to the behavioral room for at least 1h 0m 0s prior to the test for habituation

2.

Place both forepaws of the rat on an horizontal bar elevated so that the rat is in an half rearing position

3.

Measure the descent latency, defined as the time it takes for both forepaws to be removed from the bar

4.

Repeat the test three times, with a brief rest in between

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