CRISPR-Combo–mediated orthogonal genome editing and transcriptional activation for plant breeding

Changtian Pan, Yiping Qi

Published: 2023-04-20 DOI: 10.1038/s41596-023-00823-w

CRISPR-Combo

orthogonal genome editing

transcriptional activation

plant breeding

Cas9

AI 解读

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Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

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CRISPR–Cas nuclease systems, base editors, and CRISPR activation have greatly advanced plant genome engineering. However, the combinatorial approaches for multiplexed orthogonal genome editing and transcriptional regulation were previously unexploited in
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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CRISPR-Combo–mediated orthogonal genome editing and transcriptional activation for plant breeding

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