BrainSaw 50 mM pH 7.4 PB slicing buffer

Fill 2L flask with MQ water to the 2L notch. Place on magnetic stirrer and set to 1000 RPM.

Weigh 3.1g of and pour into the flask.

Weigh out 20.8g of but do not pour into flask until existing solute is dissolved.

Wait until has dissolved. Skipping this step will result in a small quantity of fine precipitate being formed.

Pour weighed out above into flask. Screw on lid and shake once to ensure no solute is stuck around the rim of the flask. Return to stirring plate at 1000 RPM.

Use PB once all solute is dissolved. Do not refrigerate: cold PB will form bubbles as it warms and this interferes with imaging.