Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Laween Meran, Lucinda Tullie, Simon Eaton, Paolo De Coppi, Vivian S. W. Li

Published: 2022-10-18 DOI: 10.1038/s41596-022-00751-1

bioengineered intestinal grafts

patient-derived organoids

bioreactor culture

decellularized scaffolds

regenerative medicine

AI 解读

Supplementary information

Supplementary Information

Supplementary Methods and Table 1

Reporting Summary

Supplementary Video 1

Bioreactor circuit components setup 1

Supplementary Video 2

Bioreactor circuit components setup 2

Supplementary Video 3

Bioreactor circuit components setup 3

Supplementary Video 4

Bioreactor circuit components setup 4

Supplementary Video 5

Preparation of scaffold

Supplementary Video 6

Mounting of scaffold

Supplementary Video 7

Injection of fibroblasts into scaffold

Supplementary Video 8

Dynamic culture circuit setup 1

Supplementary Video 9

Dynamic culture circuit setup 2

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets

Identification of cancer-related mutations in human pluripotent stem cells using RNA-seq analysis

Spiral volumetric optoacoustic tomography for imaging whole-body biodynamics in small animals

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

Tutorial: design and execution of CRISPR in vivo screens

Reproducible strategy for excisional skin-wound-healing studies in mice

CRISPR–Cas9-mediated chromosome engineering inArabidopsis thaliana

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging

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Supplementary information

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Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

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