Beetle rearing media
Yin-Tse Huang, Tina
Published: 2022-05-30 DOI: 10.17504/protocols.io.8epv59d3jg1b/v1
Abstract
ambrosia beetle rearing
Steps
1.
The recipe produces enough medium to fill approximately 36 culture tubes.(12cm)
First, add powder or solid, then add liquid
| A | B |
|---|---|
| component | amount |
| streptomycin | 0.35 g |
| Wesson's salt mixture | 1.25 g |
| yeast | 8 g |
| corn starch | 8 g |
| casein | 10 g |
| agar | 30 g |
| sucrose | 5 g |
| wheat germ oil | 2.5 ml |
| 95% ethanol | 5 ml |
| redbay sawdust | 200 g |
| deionized water | 580 ml |
2.
All ingredients were mixed thoroughly, then put cotton plugs into the mouth of the filled tubes.
3.
The culture tubes was autoclaved at 121 °C for 30 min.
4.
Put culture tubes to dry 2 night in oven.
(cotton plugs don't remove)
