Assessing biofilm inhibition and immunomodulatory activity of small amounts of synthetic host defense peptides synthesized using SPOT-array technology

Hashem Etayash, Evan F. Haney, Robert E. W. Hancock

Published: 2021-04-08 DOI: 10.1038/s41596-021-00500-w

high-throughput screening

antibiofilm activity

SPOT-array technology

host defense peptides

immunomodulatory activity

AI 解读

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Peptides are promising drug candidates because of their diversity, biocompatibility and spectrum of activities. Here, we describe a protocol for high-throughput screening of SPOT-peptide arrays to assess the antibiofilm, antimicrobial and immunomodulatory
Introduction
Materials
Procedure 1: preparing stock solutions of the SPOT peptides
Procedure 2: evaluating the biofilm inhibition activity of the SPOT peptides
Procedure 3: evaluating the immunomodulatory activity of the SPOT peptides
Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Assessing biofilm inhibition and immunomodulatory activity of small amounts of synthetic host defense peptides synthesized using SPOT-array technology

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