Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro Kasey Y. C. Lau, Gianluca Amadei, Magdalena Zernicka-Goetz Published: 2023-10-10 DOI: 10.1038/s41596-023-00891-y Embryo models Induced stem cells Gastrulation Mouse embryogenesis Stem cell assembly AI 解读 浏览 1304 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols High-throughput single-cell transcriptomics of bacteria using combinatorial barcoding Inducible mouse models of colon cancer for the analysis of sporadic and inflammation-driven tumor progression and lymph node metastasis An unbiased method for evaluating the genome-wide specificity of base editors in rice Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH Designing and executing prime editing experiments in mammalian cells Design and application of the transformer base editor in mammalian cells and mice Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting Routine culture and study of adult human brain cells from neurosurgical specimens Simultaneously measuring the methylation of parent and daughter strands of replicated DNA at the single-molecule level by Hammer-seq Spatial visualization of comprehensive brain neurotransmitter systems and neuroactive substances by selective in situ chemical derivatization mass spectrometry imaging 查看全部 Coupling of TMEM192 antibody to MyOne™ Epoxy Dynabeads™ ASAPCRN lysosomes TMEM192 Quantification of foliar polyphenolic concentration using a 96-well microtitre method fungi tannic acid phenolic Creating Bacterial Glycerol Stocks for Long-term Storage Keller's Band Counting Method with Chloroquine NCBI submission protocol for microbial pathogen surveillance NCBI submission WGS pathogen surveillance GenomeTrakr Protocol PROCI immunofluorescent staining with anti-GFP and anti-CD63 antibodies ASAPCRN LEGACY01: ADVERSE EVENTS Single-cell sequencing lymph node influenza vaccine ancestry Processing of pediatric bronchoalveolar lavage samples for single cell analysis single cell analysis BAL respiratory pediatric lung flow cytometry Nano3P-seq Protocol 查看全部 Prediction of Acute Hepatotoxicity With Human Pluripotent Stem Cell-Derived Hepatic Organoids cytochrome P450 drug testing hepatic organoid hepatotoxicity human pluripotent stem cell differentiation liver A Step-by-Step Guide for the Production of Recombinant Fluorescent TAT-HA-Tagged Proteins and their Transduction into Mammalian Cells DNA molecular assembly fluorescent protein fusion protein Ni-NTA-based protein purification protein transduction recombinant protein production tagged protein MHC II–EGFP Knock-in Mouse Model APCs light-sheet fluorescence microscopy lymphoid organs MHC II–EGFP knock-in mouse quantitative immunology SARS-CoV-2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup COVID19 COVID-19 ELISA protein expression SARS-CoV-2 serological assay Generation and Functional Characterization of Monocytes and Macrophages Derived from Human Induced Pluripotent Stem Cells differentiation functional characterization induced pluripotent stem cells macrophages monocytes Super-Resolution Stimulated Raman Scattering Microscopy with Graphical User Interface–Supported A-PoD deconvolution metabolic imaging stimulated Raman scattering super-resolution Protein Structural Modeling for Electron Microscopy Maps Using VESPER and MAINMAST cryo-EM alignment de novo protein modeling electron microscopy maps protein fitting protein structure Hepatitis E Virus: Isolation, Propagation, and Quantification HEV isolation propagation virus quantification Do more with Less: Improving High Parameter Cytometry Through Overnight Staining antibody incubation high-parameter cytometry optimization overnight staining spectral cytometry titration Isolation and Culturing of Primary Murine Chondroprogenitor Cells: A Mammalian Model of Chondrogenesis cartilage formation chondrogenesis limb bud micromass culture mouse embryo 查看全部