Artificial saliva

a.g.henry, Bjorn Bartholdy

Published: 2021-10-27 DOI: 10.17504/protocols.io.3byl4k1jzvo5/v1

Abstract

Creating an artificial saliva solution for oral biofilm growth.

This is a modified version of Sissons et al. 1991.

Mix the solution under a fumehood. It can smell pretty bad.

Also have the incubator under a fumehood, if possible. I had no such luxury...

Add 300mL distilled (or deionized) dH₂O to a 1000mL beaker, with stirring and heat 60°C .

Add:

Let the reagents completely dissolve before continuing to the next step

Add:

Add the remaining 700mL distilled (or deionized) dH₂O

Adjust to 7 with and stirring

Transfer to two 1000 ml bottles, so half of each bottle is filled.

Autoclave at 121°C , 1 Bar for 0h 15m 0s minutes

Safety information

Do NOT screw bottle caps on tightly. Loosely screw the caps on the bottles or cover the tops with foil

Once the solution has cooled, add:

Store in fridge at ca. 4°C

Occasionally test the pH to ensure it stays around 7