An end-to-end workflow for multiplexed image processing and analysis Jonas Windhager, Vito Riccardo Tomaso Zanotelli, Daniel Schulz, Lasse Meyer, Michelle Daniel, Bernd Bodenmiller, Nils Eling Published: 2023-10-09 DOI: 10.1038/s41596-023-00881-0 single-cell analysis multiplexed imaging image processing steinbock toolkit R/Bioconductor packages AI 解读 浏览 1102 PDFMetadataSupplementary information Supplementary Information Supplementary Notes 1 and 2. Reporting Summary 推荐阅读 Nature Protocols Protocols IO Current Protocols Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data ProBac-seq, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets Identification of cancer-related mutations in human pluripotent stem cells using RNA-seq analysis Spiral volumetric optoacoustic tomography for imaging whole-body biodynamics in small animals Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells Tutorial: design and execution of CRISPR in vivo screens Reproducible strategy for excisional skin-wound-healing studies in mice CRISPR–Cas9-mediated chromosome engineering inArabidopsis thaliana Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids Tracking endocytosis and intracellular distribution of spherical nucleic acids with correlative single-cell imaging 查看全部 VIRUSHUNTER™ ARTIC SARS-CoV-2 Whole Genome Sequencing Assay COVID-19 FastDNA SPIN Kit for Soil SOP for snap-freezing tissues Background control of Gotcha RCA Characterization of human immune cell subpopulations in cerebrospinal fluid using mass cytometry. The Acute Effects of Gastric Electrical Stimulation on Nucleus of the Solitary Tract Neural Activity Assessed with Electrophysiological Recording Immunofluorescence ASAPCRN Label-free quantification (LFQ) proteomic data analysis from DIA-NN output files Data analysis proteomics DIA volcano plots Welch's t-Test Transformation Protocol for BL21(DE3) Competent Cells (C2527I) T7 Expression Strain comp cells transforming for BL21(DE3) REDI-NET FF-1 FILTH FLY FIELD SAMPLING Horse fly Face fly House fly Horn fly Filth fly cattle field collection zoonotic vDNA 查看全部 greenCUT&RUN: Efficient Genomic Profiling of GFP-Tagged Transcription Factors and Chromatin Regulators CUT&RUN genome localization GFP-fusion proteins micrococcal nuclease mapping transcription factor binding genomic profiling greenCUT&RUN Highly Selective, CRISPR/Cas9-Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast CRISPR/Cas9 TAR cloning transformation-associated recombination Acute Myeloid Leukemia Minimal Residual Disease Detection: The Difference from Normal Approach acute myeloid leukemia AML flow cytometry minimal residual disease MRD The Isolation, Culture, and Cryopreservation of Human Rectus Sheath Fibroblasts abdominal wall fibroblast hernia isolation rectus sheath Assessing Lupus-Like Disease in Murine Model Systems animal models immune system immunology lupus SLE Getting Started with the IDG KMC Datasets and Tools bioinformatics data visualization disease ontology drug discovery drug targets druggable genome web applications Human Dendritic Cell Enrichment and Their Activation of T Cells MHC II–EGFP Knock-in Mouse Model APCs light-sheet fluorescence microscopy lymphoid organs MHC II–EGFP knock-in mouse quantitative immunology Live Imaging and Quantification of Neutrophil Extracellular Trap Formation automated quantification live imaging neutrophil extracellular trap Update: Induction of Inflammatory Bowel Disease in Immunodeficient Mice by Injection of Naïve CD4+ T cells (T Cell Transfer Model of Colitis) CD4+ colitis inflammatory bowel disease mouse model T cell transfer 查看全部
